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S14-18
(P)
EXPRESSION OF SEX HORMONE RECEPTORS (ANDROGEN, ESTROGEN,
PROGESTERONE) IN UROGENITAL TRACK OF THE CHILDREN: TARGET
HYPOSPADIAS
Sinan CELAYIR
1
, Aysenur CELAYIR
2
, Serdar MORALIOGLU
2
, Handan CETINER
3
and Gözde KIR
4
1) Istanbul Uni. Cerrahpasa Medical Faculty, Pediatric Surgery, Istanbul, TURKEY - 2) Zeynep Kamil Maternity and
Children's Training and Research Hospital, Pediatric Surgery, Istanbul, TURKEY - 3) Zeynep Kamil Maternity and
Children's Training and Research Hospital, Pathology, Istanbul, TURKEY - 4) Umraniye Training and Research Hospital,
Pathology, Istanbul, TURKEY
PURPOSE
Androgen (AR) and Estrogen (ER) hormones effect and play an important role in the development of penis and
hypospadias. In this study we aimed to investigate the sex hormone receptor expression - AR, ER and Progesterone(PR)
- in different types of hypospadias.
MATERIAL AND METHODS
This study was done in patients operated due to hypospadias. The biopsies were obtained from the anterior and
posterior preputium and the lateral parameatal tissue at the edge of urethral plate. The presence of AR, ER and PR
receptors were investigated. For this an avidin-biotin horseradish peroxidase technique was used to localize the
expression of AR, ER and PR receptors under a light microscope.
RESULTS
33 biopsies from 18 patients were included. There were subcoronal (n:5), penile (n:7) and penoscrotal (n:6)
hypospadias. 13 lateral parameatal tissue, 13 anterior and 7 posterior preputial biopsies investigated respectively.
16 patients underwent TIPU and 2 patients ONLAY repair. The mean age was 5.4 years.
ER receptor was positive in 87.8%, AR positive in 36.4%. Progesterone receptor was negative in all.
Lateral parameatal tissue were ER positive 69.2%, AR positive 38.5%, anterior preputium biopsy were ER positive in
100%, AR positive 30.8%, posterior preputium biopsy were ER positive 100% and AR positive 42.8% respectively.
Hypospadias Type: In subcoronal hypospadias ER were positive 80%, AR in 40%, in penile hypospadias ER was positive
in 86.7%, AR 33.3%, in penoscrotal hypospadias ER was positive in 84.6%, AR positive 38.5% respectively.
CONCLUSIONS
PR receptor was not demonstrated in sampled tissues. AR receptors were less positive than ER. There was not a
manifest correlation of AR absence in regard to the severity of hypospadias. The ER presence in penile and preputial
tissues were remarkable. As this study emphasized the dominant expression of ER receptors in children with
hypospadias, we suggest that the disrupted AR and ER receptor interaction and/or balance could play a role during the
development of external genitalia in hypospadias patients.