Abstract Book - 26th ESPU Congress, Joint Meeting with ICCS + SPU + AAP/SOU + AAPU + SFU

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S2: BASIC SCIENCE 2
Moderators: Berk Burgu (Turkey), Christian Radmayr (Austria)
ESPU Meeting on Wednesday 14, October 2015, 15:35 - 17:05
15:35 - 15:38
S2-1 (PP)
★ EFFECTIVENESS OF ARGON NOBLE GAS FOR KIDNEY PRESERVATION IN A
PRECLINICAL MODEL OF PEDIATRIC LIVING DONOR TRANSPLANTS.
Alice FAURE1, Laurie BRUZZESE2, Julia TORRENTS3, Stephane BERDAH4, Matthieu CHALOPIN5, Regis GUIEU2, Emmanuel
FENOUILLET2, Eric LECHEVALLIER6, Jean-Michel GUYS7 and Thierry MERROT7
1) Aix-Marseille university-APHM, Paediatric Urology Department, Marseille, FRANCE - 2) Aix-Marseille university-APHM,
UMR-MD2, Marseille, FRANCE - 3) Aix-Marseille university-APHM, Department of Pathology, Marseille, FRANCE - 4) Aix-
Marseille university-APHM, CERC, Marseille, FRANCE - 5) Air Liquide, Medical Gases group, Jouy En Josas, FRANCE - 6)
Aix-Marseille university-APHM, Urology Department, Marseille, FRANCE - 7) Aix-Marseille university-APHM, Pediatric
Urology Department, Marseille, FRANCE
PURPOSE
Taking into account that it is nowadays possible to prolong preservation of perishable food products under modified
atmosphere, we developed an innovative method for organ preservation. The use of noble gas to saturate a cold-storage
solution to preserve kidney graft functionality and integrity was investigated in an clinically relevant porcine preclinical
model.
MATERIAL AND METHODS
We developed a protocol based on the saturation of a cold-storage solution Celsior saturated with pure argon (Argon-
Celsior) or xenon (Xenon-Celsior) in a established pig-model of kidney autotransplantation (Faure A et al. Transplant
Proc. 2013;45:672-6). Celsior saturated with atmospheric air (Air-Celsior) was used as a control. The left kidney was
removed and Air-Celsior (n=8), Argon-Celsior (n=8) or Xenon-Celsior (n=6) was used at 4°C to flush and store the
transplant for 30h. Heterotopic autotransplantation and contralateral nephrectomy were performed. Renal function
parameters were monitored for 21 days. Survival and histological characteristics were addressed.
RESULTS
Argon-Celsior vs Air-Celsior: i) improved graft function recovery as monitored using creatinine clearance (116 ±15
ml/min vs 418.3 ±16.5 ml/min at postopeartive day 14, p=0.05), fraction of excreted sodium, and tubulopathy
duration; ii) enabled diuresis recovery 2-3days earlier; iii) improved survival (7/8 vs 3/8); iv) preserved histological
structures as explored by monitoring fibrosis, tubular lesions and inflammation. In contrast, the use of Xenon-Celsior
was detrimental and no animal survived at day-8. The positive effect of argon is not attributable to noble gasesas a
group.
CONCLUSIONS
Saturation of a preservation solution with argon improves function recovery, and hence ex vivo preservation of the
transplant during static cold-storage. The manipulation of the gas component of a preservation medium constitutes a
promising approach to improve graft preservation in clinical conditions.