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THE EFFECT OF L-ARGININE AND TADALAFIL IN RAT TESTIS ISCHEMIA
REPERFUSION INJURY
Onur KAYGISIZ
1
, Gokhun OZMERDIVEN
1
, Berna VURUSKAN
2
, Hakan KILICARSLAN
1
, Burhan COSKUN
1
, Yakup KORDAN
1
and Hakan VURUSKAN
1
1) Uludag University, Faculty of Medicine, Department of Urology, Bursa, TURKEY - 2) Uludag University, Faculty of
Medicine, Department of Pathology, Bursa, TURKEY
PURPOSE
We aimed to examine the effect of L-arginine and tadalafil for prevention of ischemia reperfusion injury after testis
torsion in rats.
MATERIAL AND METHODS
A total of 40 adult, male Sprague Dawley rats were divided into 5 groups randomly. No testicular torsion was performed
in control (1st) group and 3 hours of left testicular torsion was performed in study groups. No drug was applied in the
2nd group, L-arginine was injected in the 3th group, tadalafil was injected in the 4th group, L-arginine and tadalafil
together were injected in the 5th group. All drugs were injected intraperitoneally 30 minutes before the detorsion. Then
left testis was untwisted for four hours of reperfusion. Bilateral testes were removed. For biochemical examination, lipid
peroxidation (LPx) and glutathione (GSH) activities were examined in testicular tissue. Spermatogenesis was evaluated
with Johnsen's score.
RESULTS
Ipsilateral testis: LPx levels of 2nd group were found to be significantly higher than all other groups in ipsilateral testis
(p<0.001). GSH levels revealed similar between groups (p=0.021).Johnsen's score of 2nd group was significantly lower
than all other groups (p<0.001).Johnsen's score of 5th group was significantly higher from Johnsen's score of 3rd and
4th groups.
Contralateral testis: LPx levels of 2nd group were significantly higher than others(p=0.001).4th group level was
significantly different than results of control and 5th group. GSH levels of 5th group were significantly higher than other
groups. Mean Johnsen's score of 2nd group was significantly lower than all others(<0.001).Johnsen's score of the 4th
group was significantly lower than Johnsen's score of 5th group.
CONCLUSIONS
L-arginine, tadalafil and combination of these two molecules showed protective effect against ischemia/reperfusion
injury.