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PREVENTION OF RENAL SCARRING IN ACUTE PYELONEPHRITIS IN THE RAT BY
PROPHYLATIC PROBIOTIC ADMINISTRATION
Nastaran SABETKISH, Shabnam SABETKISH and Abdol-Mohammad KAJBAFZADEH
Children's Hospital Medical Center, Pediatric Urology Research Center, Section of Tissue Engineering and Stem Cells
Therapy, Tehran, IRAN (ISLAMIC REPUBLIC OF)
PURPOSE
To present the protective effect of probiotic regimen as a prophylaxis treatment in renal scaring by increasing the
immune response in a rat model of acute pyelonephritis.
MATERIAL AND METHODS
Twelve rats were divided into 3 equal groups. All groups received direct inoculation of10
9
colony-forming units of
Escherichia coli (E. coli, ATCC 25922 strain) into the right kidney. Group I was served as control. Group II: prophylactic
probiotic regimen 1 month before and 2 months after E. coli injection. Group III: therapeutic probiotic regimen 2
months after E. coli injection. Body temperature was recorded after bacterial inoculation.Technetium-99m-DMSA renal
scan, histopathological evaluations, concentrations of CA19-9, Ig-A, blood urea nitrogen, and creatinine were performed
1 and 2 months post-injection.
RESULTS
It took an average of 4.2 ± 1.1 hours between the injection and onset of fever. In group II, this period was longer (7.5
± 1.4). Probiotic administration resulted in reduction of interstitial fibrosis, tubular, and glomerular atrophy in group II.
Two months after the injection, Technetium-99m-DMSA renal scan showed that right kidney reached to near normal
cortical configuration in group II (48%) as compared to control group (38%) and group III (41%). In group II, CA19-9
level was lower, while Ig-A level was in higher concentrationas compared to other groups.
CONCLUSIONS
These findings may pave the road for prevention of renal scarring in children with vesicoureteral reflux and recurrent
pyelonephritis. However, its application after the onset of fever may not be as efficient as its prophylactic
administration.