Abstract Book - 26th ESPU Congress, Joint Meeting with ICCS + SPU + AAP/SOU + AAPU + SFU

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16:05 - 16:08
S2-11 (PP)
USE OF MRI DIFFUSION SEQUENCE TO QUANTIFY KIDNEY PARENCHYMA
IMPAIRMENT IN A PARTIAL URETERAL OBSTRUCTION MOUSE MODEL
Maguelonne PONS1, Liza ALI1, Simon, A LAMBERT2, Benjamin LEPORC3, Marianne ALISON4, Michel PEUCHMAUR5, Ulrich
BLANK6 and Alaa EL GHONEIMI 1
1) Hopital Robert Debré, APHP, INSERM UMR 1149, Université Paris 7 Diderot, Sorbonne Paris Cité, Department
paediatric surgery and urology, Laboratoire d'excellence INFLAMEX, Paris, FRANCE - 2) BHF Centre of Excellence,King's
College London, King's Health Partners,, Division of Imaging Sciences and Biomedical Engineering, London, UNITED
KINGDOM - 3) INSERM UMR 1149, Université Paris 7 Diderot, Sorbonne Paris Cité,, Imaging, Clichy, FRANCE - 4)
Hopital Robert Debré, APHP, Université Paris 7 Diderot, Sorbonne Paris Cité, Department of Paediatric Radiology, Par,
FRANCE - 5) Hopital Robert Debré, APHP, Université Paris 7 Diderot, Sorbonne Paris Cité, Department paediatric
anatomopathology, Paris, FRANCE - 6) INSERM UMR 1149, CNRS ERL8252, Université Paris 7 Diderot, Sorbonne Paris
Cité, Laboratoire d'excellence INFLAMEX, Paris, FRANCE
PURPOSE
Obstructive nephropathy constitutes a major cause of paediatric renal progressive disease. Fibrotic lesions in the kidney
can be reproduced by a model of partial ureteral obstruction (UUOp). We have proved the importance of morphological
impairment evaluation by MRI in UUOp. To go further we propose to use intravoxel incoherent motion (IVIM) diffusion
sequence to characterize kidney parenchyma impairment.
MATERIAL AND METHODS
The diffusion coefficient (Dslow), the perfusion coefficient (Dfast) and the perfusion fraction (f) were extracted from
IVIM data acquired on a 7T preclinical system using a Matlab homemade software. The imaging method was validated
on 10 sham wild type (WT) mice. Then 6WT mice were subjected to UUOp at day 3 of life. At day 75, mice underwent
MRI examinations.
RESULTS
Extracted diffusion parameters from IVIM were similar in both kidneys of sham WT mice. Mean values of Dslow, Dfast
and f were respectively 1.08±0.31mm2.s-1, 78.67±70.58mm2.s-1 and 30±6% in the right kidney and
1.06±0.32mm2.s-1, 88.39±65.63mm2.s-1, and 30±7% in the left kidney. For UUOp mice, right operated kidneys with
important morphological impairment on MRI and histologically proven fibrosis, showed a decreaseof Dslow (right
kidney:0.82±0.13mm2.s-1, left:0.98±0.08mm2.s-1), whereas for the less-injured operated kidneys no difference was
observed (right kidney:0.98±0.02mm2.s-1, left:0.98±0.04mm2.s-1).
CONCLUSIONS
The IVIM sequence has been validated for the first time on mice kidney at 7T. The preliminary results on UUOp mice
show the benefit of IVIM to stage kidney parenchyma fibrosis. The study will be enhanced by increasing the number of
operated mice.