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BSP: BASIC SCIENCE (posters)
ESPU Meeting
BSP-1
(P)
NONAUTOLOGOUS MESENCHYMAL STEM CELL TRANSPLANTATION IN
EXPERIMENTAL MODEL OF ACUTE PYELONEPHRITIS FOR REPAIR OF RENAL
SCARRING
Abdol-Mohammad KAJBAFZADEH, Shabnam SABETKISH and Nastaran SABETKISH
Children's Hospital Medical Center, Pediatric Urology Research Center, Section of Tissue Engineering and Stem Cells
Therapy, Tehran, IRAN (ISLAMIC REPUBLIC OF)
PURPOSE
We compared the therapeutic effects of nonautologous adipose-derived mesenchymal stem cell (ADMSC) injection,
obtained from the nape and perirenal adipose tissue of male rabbits for repair of renal scarring in a rabbit model of
acute pyelonephritis.
MATERIAL AND METHODS
Twelve female rabbits were divided into 3 equal groups. Group I (control): direct inoculation of 5 ×109 colony-forming
units of Escherichia Coli (E. Coli, ATCC 25922 strain) into the right kidney. Group II: injection of nonautologous ADMSCs
(8.5×106) obtained from the perirenal adipose tissue of male rabbits into the subcapsular space 6 weeks after injection
of E. Coli. Group III: injection of nonautologous ADMSCs obtained from the nape of male rabbits with the same method
of group II. Body temperature was recorded after bacterial inoculation. Technetium-99m-DMSA renal scan and
histopathological evaluations were performed 1 and 2 months post-injection in all groups.
RESULTS
It took an average of 14.2 ± 1.1 hours between the injection and onset of fever. Injection of ADMSC resulted in
reduction of interstitial fibrosis, tubular, and glomerular atrophy in groups II and III as compared to control group.
However, the histopathological amelioration was significantly better in group II. Two months after the injection,
Technetium-99m-DMSA renal scan showed that right kidney reached to near normal cortical function in group II(47%)
and group III (44%), as compared to control group (36%).
CONCLUSIONS
These findings propose that injection of ADMSCs obtained from the perirenal adipose tissue may modify the
histopathological characteristics and renal function after acute pyelonephritis.