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BSP-3

(P)

INVESTIGATION OF THE EFFECT OF PENILE TOURNIQUET APPLICATION TO

BACTERIAL ADHESION

Ozlem BOYBEYI

1

, Birgul KACMAZ

2

, Esra ARAT

3

, Pinar ATASOY

4

, Ucler KISA

5

, Yasemin DERE GUNAL

6

, Mustafa Kemal

ASLAN

6

and Tutku SOYER

7

1) HACETTEPE UNIVERSITY MEDICAL FACULTY, DEPARTMENT OF PEDIATRIC SURGERY, Ankara, TURKEY - 2) KIRIKKALE

UNIVERSITY, MEDICAL FACULTY, DEPARTMENT OF CLINICAL MICROBIOLOGY AND INFECTIOUS DISEASES, Kirikkale,

TURKEY - 3) KIRIKKALE UNIVERSITY, SCIENCE FACULTY, DEPARTMENT OF BIOLOGY, Kirikkale, TURKEY - 4) KIRIKKALE

UNIVERSITY, MEDICAL FACULTY, DEPARTMENT OF PATHOLOGY, Kirikkale, TURKEY - 5) KIRIKKALE UNIVERSITY,

MEDICAL FACULTY, DEPARTMENT OF BIOCHEMISTRY, Kirikkale, TURKEY - 6) KIRIKKALE UNIVERSITY, MEDICAL

FACULTY, DEPARTMENT OF PEDIATRIC SURGERY, Kirikkale, TURKEY - 7) HACETTEPE UNIVERSITY, MEDICAL FACULTY,

DEPARTMENT OF PEDIATRIC SURGERY, Ankara, TURKEY

PURPOSE

Penile tourniquet (PT) application might decrease local tissue perfusion and alter cell integrity causing increased

bacterial adhesion. Experimental study was conducted to evaluate the effect of PT to bacterial adhesion.

MATERIAL AND METHODS

Fifty-six rats were allocated into control group (CG, n=7), sham group (SG, n=21), PT group (PTG, n=21). No

intervention was done in CG. A 5 mm-length urethral repair was performed in SG and PTG. In PTG, 10min-duration PT

was applied during procedure and tissue oxygenation monitor (MoorVMS-OXY) was used to achieve same degree of

ischemia in all subjects. SG and PTG were allocated into 3 subgroups according to time of tissue harvesting; 1 hour (SG-

1, PTG-1), 24 hour (SG-2, PTG-2), and 72 hour (SG-3, PTG-3). Samples were examined biochemically (nitric-oxide-NO,

malondialdehyde-MDA, myeloperoxidase-MPO, TNF-α, IL-1β), histopathologically (wound healing scores) and electron

microscopically (bacterial adhesion screening).

RESULTS

MDA, MPO, TNF-α, IL-1β levels were significantly decreased in SG and PTG compared to CG (p<0.05). However, there

was no difference between SG and PTG (p>0.05). The mean values of biochemical markers did not show significant

difference in early and late harvested samples (p>0.05). Wound healing scores were higher in SG than CG and in PTG

than CG and SG (p<0.05). The scores were significantly altered in samples with late harvesting time (p<0.05). Electron

microscopic examination showed higher bacterial adhesion in PTG than CG and SG.

CONCLUSIONS

PT did not cause inflammatory marker exhaustion and oxidative injury after urethral repair. However, tourniquet

application alters histopathological findings of wound healing and causes increased bacterial adhesion. Since these

adverse findings became prominent at 72 hour after application, we can suggest that PT may cause postoperative

complications such as wound dehiscence and infection.