Abstract Book - 26th ESPU Congress, Joint Meeting with ICCS + SPU + AAP/SOU + AAPU + SFU

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BSP-7 (P)
DEVELOPING TESTIS IS HYPOXIC. EMBRYONIC AND EARLY POSTNATAL TESTIS
IS LESS VULNERABLE FOR HYPOXIA WHEN COMPARED TO AT LATER STAGES OF
LIFE.
Onur TELLI1, Nurullah HAMIDI2, Baris ESEN2, Gulnur Gollu BAHADIR3, Duygu KANKAYA4, Tarkan SOYGUR1 and Berk
BURGU1
1) Ankara University, Pediatric Urology, Ankara, TURKEY - 2) Ankara University, Urology, Ankara, TURKEY - 3) Ankara
University, Pediatric Surgery, Ankara, TURKEY - 4) Ankara University, Pathology, Ankara, TURKEY
PURPOSE
Developing tissues are hypoxic to generate aimed to determine presence of hypoxia and angiogenetic
pattern in developing the developing testis is hypoxic,we hypnotized that embryonic and early postnatal
testicular tissue is less vulnerable for hypoxia when compared to testis at later stages of life.
MATERIAL AND METHODS
We demonstrated the presence of hypoxia in the developing testis by means of a HIF1-alpha and piminidazole and the
angiogenetic pattern by CD31 in different embryonic stages (E16,E18,E20 and postnatal day1)in mice model.Whole
testes from mild-type mice at embryonic day 16, 18 and postnatal D1 and testicular tissues from one-week, one-month
and post-pubertal mice were cultured in %20 and %3O2atmospheres by the technique that we have previously
reported.For each group after 1, 3 or 6 days explants were evaluated in terms of apoptosis(tunnel-test) and
proliferation(Ki67).
RESULTS
We found that HIF1-alpha and angiogenetic origins were spatiotemporally co-localized.The embryonic,postnatal D1 and
one-week %3O2 explants were significantly less vulnerable to hypoxic environment after 3 and 6 days when compared
to one month and adult hypoxic conditions the embryonic and postnatal testicular tissues up to 1 week
revealed a significantly higher proliferative and a lower apoptotic index when compared to explants of older
age(p=0.012;p=0.009,respectively).
CONCLUSIONS
Hypoxia exists widely in the developing embryonic and even in postnatal D1 testis.Angiogenesis continues even in the
first days of postnatal life, which initiates from the tiny hilar peritubuler explants (even up to 6 days)
embryonic testes and postnatal D1 and one-week old testicular tissues are more resistance to hypoxic damage(shown
by proliferative and apoptotic indices) when compared to one month and adult testicular tissues.This initiates the
question that,as the neonatal testes is less affected from hypoxia a longer time frame for intervention can potentially be
considered.Assuming that viability can still be possible after long hypoxic periods surgeons should still plan surgical
detorsion even for late admitting infant patients with long a history.