40 / 492
BSP-5
(P)
PREVENTIVE EFFECT OF AMINOGUANIDINE ON RENAL SCAR IN A RAT MODEL OF
PYELONEPHRITIS
Bahadir CALISKAN
1
, Bulent UYSAL
2
, Tuncer CAYCI
3
, Ayhan OZCAN
4
, Orhan BEDIR
5
, Ilhami SURER
6
and Ahmet GUVEN
6
1) Gulhane Military Medical Academy Hospital, Pediatric Surgery, Ankara, TURKEY - 2) GULHANE MILITARY MEDICAL
FACULTY, DEPARTMENT OF PHYSIOLOGY, Ankara, TURKEY - 3) GULHANE MILITARY MEDICAL FACULTY, DEPARTMENT
OF BIOCHEMISTRY, Ankara, TURKEY - 4) GULHANE MILITARY MEDICAL FACULTY, DEPARTMENT OF PATHOLOGY,
Ankara, TURKEY - 5) GULHANE MILITARY MEDICAL FACULTY, DEPARTMENT OF MICROBIOLOGY AND CLINICAL
MICROBIOLOGY, Ankara, TURKEY - 6) GULHANE MILITARY MEDICAL FACULTY, DEPARTMENT OF PEDIATRIC SURGERY,
Ankara, TURKEY
PURPOSE
Acute Pyelonephritis (APN) in children results with scar formation as a result of inflammatory and oxidative/nitrosative
stress processes. This study aimed to evaluate the effect of Aminoguanidine (AG), an inducible nitric oxide synthase
(iNOS) inhibitor, on the renal function, morphology and biochemical parameters of oxidative stress in an experimental
model of APN in rats.
MATERIAL AND METHODS
Forty-rats were divided equally into five groups as control, APN, APN+Antibiotic, APN+AG, and APN+Antibiotic+AG. APN
was induced by 0.1 ml of freshly prepared Escherichia coli (ATCC 25922) solution containing 10
10
colony-forming unit/ml
into the kidney while control group was administered 0.9 % NaCl solution. Treatment was begun 72 h after bacterial
inoculation. Control and APN groups were given 0.9% NaCl solution, treatment groups were given antibiotic and/or AG
for five consecutive days. Seven days later, after decapitation trunk blood was collocted, both kidneys were harvested
were for biochemical and histopathological evaluation.
RESULTS
E. coli -induced APN increased renal dysfunction and oxidative stress parameters (malondialdehyde and protein carbonyl
content) and antioxidant enzyme parameters (Superoxide dismutase and glutathione peroxidase). NO
x
(nitrite/nitrate)
were also raised in APN group. Either antibiotherapy or AG markedly ameliorated renal dysfunction, the antioxidant
status of the kidneys and histopathological injuries. The combination of antibiotherapy and AG was significantly more
effective than either of the treatment modalities alone. Histopathologic evaluation showed correlation with laboratory
results.
CONCLUSIONS
AG might be considered as an adjuvant therapy to classical antibiotherapy to prevent renal inflammation and fibrosis in
APN.