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15:23 - 15:26
S27-3
(PP)
WHAT IS THE RISK OF URINE CONTAMINATION FOR NON-TOILET-TRAINED AND
UNCIRCUMCISED BOYS?
Quentin BALLOUHEY
1
, Laurent FOURCADE
1
, Jérôme CROS
2
, Pauline CLERMIDI
1
, Victor LESCURE
1
, Fabien GARNIER
3
and
Vincent GUIGONIS
4
1) Limoges university hospital, Pediatric surgery, Limoges, FRANCE - 2) Limoges university hospital, Pediatric
anesthesiology, Limoges, FRANCE - 3) Limoges university hospital, Bacteriology, Limoges, FRANCE - 4) Limoges
university hospital, Pediatric nephrology, Limoges, FRANCE
PURPOSE
Contamination during urine collection makes diagnosing urinary tract infections (UTI) difficult, particularly for young
children. Few studies are available for uncircumcised and non-toilet-trained boys. The aim of this study was to evaluate
urine contamination and to assess any differences in the initial and midstream urine samples from non-toilet-trained,
uncircumcised boys.
MATERIAL AND METHODS
A prospective diagnostic study between early and midstream urine samples was conducted on asymptomatic patients in
a pediatric surgery department. The clean void method was performed in non-toilet-trained boys under general
anesthesia. The exclusion criteria were circumcision, older than three years of age, recent antibiotics treatment, and
recent UTI. A urinalysis and quantitative culture were taken and compared between early and midstream urine samples.
A positive culture was defined as a colony count of more than 5 × 104 CFU/mL.
RESULTS
Forty-four patients were enrolled in the study, and 31 satisfactory samples were obtained. The high contamination rate
found in the early stream (n = 16; 51%) versus midstream (n = 5; 16%) was statistically significant (p < 0.01 by
Fisher's exact test). The positive culture from the early stream sample was statistically associated with a lower age (p =
0.02).
CONCLUSIONS
The clean void method in non-toilet-trained, uncircumcised boys provides high-contaminated urine samples for both
early and midstream urine samples. Therefore, caution is required for the positive diagnosis of UTI in this population
with the use of bag samples or clean void method.