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BSP-17
(P)
A STUDY OF TWO COMMON PROINFLAMMATORY POLIMORPHISMS TNF-ALPHA
G(-238)A AND IL10 ( RS1800871) WITH VESICOURETERAL REFLUX RISK IN
RUSSIAN PEDIATRIC POPULATION.
Dmitry SHAKHNOVSKIY
1
, Sergei ZORKIN
1
, Aleksandr PUSHKOV
2
and Kirill SAVOST'ANOV
2
1) Scientific centre of children health, Urology, Moscow, RUSSIAN FEDERATION - 2) Scientific centre of children health,
Molecular genetics, Moscow, RUSSIAN FEDERATION
PURPOSE
The aim of this study was to estimate whether the most common functional genetic polymorphisms in the promoter
region of TNF-alpha and IL10 genes are implicated in the development of vesicoureteral reflux (VUR), because a vast
literature indicates that genetic variations in this genes play a significant role in pathogenesis of VUR
MATERIAL AND METHODS
The Real-time PCR, using fluorescent TaqMan probes were applied for analyzing the polymorphic sites of G(-238)A of
TNF-alpha and C(-819)T of IL10 genes in 109 healthy controls and 97 children with VUR.
RESULTS
We didn't reveal a statistically significant association of polymorphism G(-238)A of TNF-alpha gene with VUR reflux.
However, we have a tendency to increase allele A and genotype GA of this polymorphism in children with VUR
(OR=1,94, p=0,090). No significant association was found between C(-819)T polymorphism of IL10 and VUR.
CONCLUSIONS
Although the present study didn't reveal an association of two common proinflammatory cytokine gene polimorphisms
TNF-alpha G(-238)A and IL10 ( rs1800871) and vesicoureteral reflux risk, analysis of results suggests the need for
further research of association of other pro-inflammatory cytokines polymorphic markers on a larger sample size. The
data obtained will be an important step in identifying the pathogenic mechanisms of VUR. To our knowledge such
studies were not previously performed in Russian population and lack of statistically significant associations might be
caused by genetic heterogeneity of the sample.