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BSP-19
(P)
NEW INDICATORS IN BLUNT RENAL TRAUMA: NGAL, KIM-1 AND IL-18
Unal BAKAL
1
, Mehmet SARAC
1
, Dilara KAMAN
2
and Ahmet KAZEZ
1
1) FIRAT UNIVERSITY, PEDIATRIC SURGERY, Elazig, TURKEY - 2) FIRAT UNIVERSITY, MEDICAL BIOCHEMISTRY, Elazig,
TURKEY
PURPOSE
We evaluated the changes of urinary and serum NGAL (Neutrophil gelatinase-associated lipocalin), KIM-1 (Kidney injury
molecule-1), IL-18 (Interleukin-18) and Cystatin-C levels in blunt renal trauma in rats.
MATERIAL AND METHODS
Twenty Sprague-Dawley rats were studied in three groups. In the Sham group, left kidney exploration was made. In the
Trauma group, after left kidney exploration, a 20 g weight was dropped on to kidneys. Third group was Control. To
analysis and determination of NGAL, KIM-1, IL-18, Cystatin-C and urine creatinin levels, urine and serum was collected
in metabolic cages at the beginning and 12-24., 36-48., 60-72. posttraumatic hours. Results were evaluated with Mann-
Whitney U and Kruscall-Wallis tests.
RESULTS
Macroscopic examinations of traumatizated kidneys revealed grade II and III injury. Hematuria was observed in all rats
in the trauma group. Serum and urine indicators were not change in Control group. Serum IL-18 and urine kreatinine
were elevated in the Sham group. While NGAL, KIM-1 and IL-18 were found to be significantly higher than their base
levels in urine, NGAL, IL-18 and Cystatin-C were found to be significantly higher than their base levels in serum.
CONCLUSIONS
Both of KIM-1 and IL-18 which were elevated in urine and NGAL which was elevated serum and urine were shown early
diagnostic and non-invasive laboratory parameters with blunt renal trauma in rats. We need more and clinical studies at
that point.